ART Clinic and ART laboratory should operate, according to ICMR guidelines and should reflect modern awareness of professional and commercial environment, and should embrace spermfuge. To maintain ambient quality there are certain vital parameters like particle count, filter integrity test, air velocity, temperature, humidity and CO2 for incubators which need to be kept under a constant check as they can harm the gametes and embryos, ultimately compromising the results in any ivf lab. http://www.shivaniivf.com